Professor, Chemistry
B.S. in Chemistry and Classics, Williams College, 1977
Ph.D. in Biophysical Chemistry, U.C. Berkeley, 1984
Office: Seaver 315
Phone: (310) 338-2943
email: jroe@lmu.edu
Classes Typically Taught:
General Chemistry; Physical Chemistry; Biochemistry; Bio
Inorganic Chemistry
Research Interests:
The roles of metal ions in biology; protein structure;
calorimetry of biomolecules.
Recent Publications:
"Familial Amyotrophic Lateral Sclerosis-associated
Mutations Decrease the Thermal Stability of Distinctly Metallated
Species of Human Copper/Zinc Superoxide Dismutase"
Rodriguez, J.A.; Valentine, J.S.; Eggers, D.K.; Roe, J.A.;
Tiwari, A;Brown, R.H. Jr; and Hayward, L.J. (2002)J. Biol. Chem,
277 (18), p. 15932.
"Whole Yeast Cells Containing Mutant Copper,Zinc-Superoxide
Dismutase Associated with hfals Demonstrate Increased Peroxidase
Activity In Vivo." Wiedau-Pazos, M; Moy, V.; Goto, J.j.;
Gralla, E.B.;Valentine, J.S.; Roe, J.A. Free Radicals Biology and
Medicine (2002) 32, 169-174.
"Chemistry" in The Volume Library (2000 ed), Rollie J.
Myers, Jr. and James A. Roe, Vol 1, pp. 475-508, Southwestern
Press, Nashville
"Mutations in Copper-Zinc Superoxide Dismutase that cause
Amyotrophic Lateral Sclerosis Alter the Zinc Binding Site and the
Redox Behavior of the Protein," Lyons, T.J.; Liu, H.; Goto,
J.J.; Nersissian, A; Roe, J.A.; Graden, J.A.; Café, C.; Ellerby,
L.M.; Bredesen, D.E.; Gralla, E.B.; Valentine, J.S. J. Proc.
Natl. Acad. Sci USA (1996) 93, 12240-12244.
"Altered Reactivity of Superoxide Dismutase in Familial
Amyotrophic Lateral Sclerosis." Wiedau-Pazos, M.; Goto,
J.J.; Rabizadeh, S.; Gralla, E.B.; Roe, J.A.; Lee, M.K.;
Valentine, J.S.; Bredesen, D.E. Science (1996) 271, 515-518.
"Unusual Trigonal-Planar Copper Configuration Revealed in
the Atomic Structure of Yeast Copper-Zinc Superoxide
Dismutase." Ogihara, N.L.; Parge, H.E.; Hart, P.J.; Weiss,
M.S.; Goto, J.J.; Roe, J.A.; Valentine, J.S.; Eisenberg, D.;
Tainer, J. A. Biochemistry (1996) 35, 2316-2321.
"New Type 2 Copper-Cysteinate-Proteins. Copper Site
Histidine-to-Cysteine Mutants of Yeast Copper-Zinc Superoxide
Dismutase." Lu, Y.; Roe, J.A.; Bender, C.J.; Peisach, J.;
Banci, L.; Berini, I.; Gralla, E.B.; Valentine, J.S. Inorganic
Chemistry (1996) 35, 1692-1700.
"Role of the Bridging Histidyl Imidazolate Ligand in Yeast
Copper-Zinc Superoxide Dismutase."Graden, J.A.; Ellerby,
L.M.; Roe, J.A.; Valentine, J.S. J. Inorg. Chem. 116, 9743-9744
(1994).
"Metalloprotein Ligand Redesign: Characterization of the
Copper-Cysteinate Proteins Derived from Yeast Copper-Zinc
Superoxide Dismutase," Lu, Y.; Roe, J.A.; Gralla, E.B.;
Valentine, J.S. in "Bioinorganic Chemistry of Copper,"
Karlin, K.D. & Tyeklar, Z., eds., pp. 64-77 (Chapman &
Hall, New York, 1993).
"Thrombin-Binding DNA Aptamer Forms a Unimolecular
Quadruplex Structure in Solution," Macaya, R. F.; Schultze,
Smith, F.W.; Roe, J.A.; Feigon, J. Proc. Natl. Acad. Sci USA 90,
3745-3749 (1993).
"Construction of a 'Blue' Copper site at the Native Zinc
Site of Yeast Copper-Zinc Superoxide Dismutase," Lu, Y.;
LaCroix, L.B.; Lowery, M.D.; Solomon, E.I.; Bender, C.J.;
Peisach, J.; Roe, J.A.; Gralla, E.B.; Valentine J.S. J. Am. Chem.
Soc. 115, 5907-5918 (1993).
"Enhanced Oxidation of Aniline Derivatives by Two Mutants of
Cytochrome c Peroxidase at Tryptophan 51," Roe, J.A.;
Goodin, D.G. J. Biol. Chem. 268, 20037-20045 (1993).
"The Redesign of a Type II Copper Protein into a Type I:
Construction and Characterization of Yeast Cu,Zn Superoxide
Mutants," Lu, Y.; Gralla, E.B.; Roe, J.A.; and J.S.
Valentine. J. Am. Chem. Soc. 114, 3560-3562 (1992).
"Amino Acid Substitutions at TRP-51 of Cytochrome c
Peroxidase: Effects on Coordination, Species Preference for
Cytochrome c, and Electron Transfer," Goodin, D.B.;
Davidson, M.G.; Roe, J.A.; Mauk, G.A.; and M. Smith.,
Biochemistry 30, 4953-4962 (1991).
"Complexometric Titration of Protein Bound Metal Ions: A
Method for Determining Binding Constants," Roe, J. A.; and
J. S. Valentine, Anal. Biochem. 186, 31-40 (1990).
"Silver Binding Properties of Bovine Cuprozinc Superoxide
Dismutase," Roe, J. A.; Peoples, R.; Scholler, D.; and J. S.
Valentine, J. Am. Chem. Soc. 112, 1538-1545 (1990).
"Differential Scanning Calorimetry of Cu,Zn-Superoxide
Dismutase, the Apoprotein, and Its Zinc-Substituted
Derivatives," Roe, J. A.; Butler, A.; Scholler, D. M.;
Valentine, J. S.; Marky, L.; and K. J. Breslauer. Biochemistry
27, 950-958 (1988).
"Magnetic and Spectroscopic Characterization of an Iron
Porphyrin Peroxide Complex. Peroxoferrioctaethylporphyrin
(1-)," Burstyn, J. N.; Roe, J. A.; Mikstal, A. R.; Shaevitz,
B. A.; Lang, G.; and J. S. Valentine. J. Am. Chem. Soc. 110,
1382-1388 (1988).
"Studies of Copper-Zinc Superoxide Dismutase: The Role of
Zinc and of the Essential Arginyl Residue," Valentine, J.
S.; Roe, J.A.; Butler, A.; and D. Mota de Freitas, in
"Biological & Inorganic Copper Chemistry", vol. I,
Eds., Karlin, K. D. and J. Zubieta, pp. 53-60, Adenine, New York
(1985).
PRESENTATIONS
"Investigation of the Thermal Stability of Streptomyces
seoulensis NiSOD." Niemoth Anderson, J. D.; , Rodriguez, J.
A.; Lee, J.-W., Roe, J. A.; Yim.,Y.-I.; Cabelli, D.E.; Valentine,
J.S.; Sa-Ouk Kang, S.-O.; Maroney, M. J. National Meeting of the
American Chemical Society held in San Francisco, March 26-30,
2000.
"Purification, Metal Ion Content, and Thermodynamic
Stability of 14 Mutant Superoxide Dismutases Associated with
Familial ALS." Hayward, L. J.; Rodriguez, J; Jang, G;
Cabelli, D.E.; Roe, J.A., Valentine, J.S.; Brown, R.H., Jr. The
11th Intl Symposium on ALS/Motor Neuron disease. Arhus,Denmark,
December 4-6, 2000.
"Loss of Metal Ion Binding Specificity in Mutant CuZnSOD
Associated FALS ." Goto, J.J; Roe, J.A.; Gralla, E.B.;
Cabelli, D.E.; Valentine, J.S. 6th Annual Oxygen Society Meeting,
New Orleans, LA: November 19-22, 1999.
"Air Oxidation of DOPA Catalyzed by Tripodal-Ligand
Complexes." With M. Mandac (LMU’96) and L. Cordero
(LMU’96). Metals In Biology Gordon Research Conference,
January 21-25, 1996: Ventura, CA
"The Effect of Metal-Ion Binding on the Thermal Stability of
Proteins." The Pacific Conference, Days Inn, Fullerton, CA,
October 5, 1995.
"When FTNMR Came to LMU." Invited Speaker at the NSF
Faculty Enhancement Workshop, Kingston, RI, July 25, 1995.
"Calorimetric Studies of the Binding of Metal-Ions to
Concanavalin A." Presentation to the Gordon Conference on
Metals in Biology, Ventura, CA, January 24, 1995.
"Differential Scanning Calorimetry of the Plant Lectin,
Concanavalin A." Presentation to the 1994 Council on
Undergraduate Research Symposium held at Bates College, Lewiston,
Maine, June 25, 1994.
"Spectroscopic and Calorimetric Studies of Metal-Substituted
Myohemerythrins." Presentation to the Gordon Conference on
Metals in Biology, Ventura, CA, January 23, 1994.
"Ligand Redesign of Yeast Copper-Zinc Dismutase:
Substitution of the Bridging Histidine Residue with
Alanine," at the Sixth International Conference on
Bioinorganic Chemistry, San Diego, August 23, 1993, co-authored
with Janet Graden et al.
"Spectroscopic and Calorimetric Studies of Metal-Substituted
Myohemerythrins," Martins, L.; Dait, F.; Phenjati, K.;
Ellis, W.R.,Jr.; Roe, J.A., at Sixth International Conference on
Bioinorganic Chemistry, San Diego, August, 23 1993.
"Cupric EPR as a Probe of the Metal Binding Sites of Mutants
Derived from Yeast Copper-Zinc Superoxide Dismutase," at The
Pacific Conference, Pasadena Hilton Hotel, October 22, 1993.
"Undergraduate Research in the Department of Chemistry and
Biochemistry of Loyola Marymount University: How Instrumentation
Drives Research," Council on Undergraduate Research
Conference, Hope College, Holland, MI, July 10, 1992..
"The Differential Scanning Calorimetry of
Myohemerythrin," with K. Phenjati’94, L. Martins, and
W. Ellis: The Sixth Symposium of the Protein Society, San Diego,
CA, July 24-29, 1992.
"The Binding of Silver Ions to the Active Site of
Cu,Zn-Superoxide Dismutase. Pacific Conference on Chemistry and
Spectroscopy, Irvine, CA, October 1987.
"Kinetic Aspects of the Differential Scanning Calorimetry of
Cu,Zn-Superoxide Dismutase," San Francisco State University
(October 1986) and Jackson State University (November 1986).
"Differential Scanning Calorimetry of Cu,Zn-Superoxide
Dismutase, the Apoprotein, and Its Zinc-Substituted
Derivatives," The 40th Calorimetry Conference, Asilomar
(August 1985).
PRESENTATIONS BY RESEARCH STUDENTS
"Paramagnetically Shifted Nuclear Magnetic Resonance
Spectroscopy of Cobalt Substituted Conconavalin A and Model
Complexes," with Va Cooc ‘97, presented at the 1997
Southern California American Chemical Society Undergraduate
Research Conference, held at Cal State Northridge, April 5, 1997.
Biomimetic Compounds: Cu(II) Complexes of Tris[2-(3,5-Dimethyl
-pyrazoyl)Methyl]Amine and
Tris[2-(3,5-Dimethylpyrazoyl)Ethyl]Amine." A presentation of
Maureen Mandac ‘96 to the Southern California Conferenceon
Undergraduate Research held at The Claremont Colleges, Calremont,
CA November 18, 1995.
"Air Oxidation of DOPA Catalyzed by a Tripodal Ligand
Complex of Copper." A Presentation by Leah Cordero ’96
to the Southern Caifornia ACS Undergraduate Research Conference
held at Cal State Fullerton, April 1, 1995.
3. Grant Proposal: Proposal to Renew a Research Corporation
Cottrell Award to support a student researcher and the principal
investigator and to obtain supplies for the summers of 1996 and
1997. Total amount requested: $22,000.00.
"Calorimetric Studies of Metal-Substituted Concanavalin
A." A Presentation by Isabel Markl ‘95 to the 2nd
Southern California Conference on Undergraduate Research held at
Cal Tech, Pasadena, CA., 19 November 1994.
"Differential Scanning Calorimetry of Myohemerythrin,"
presented by Krisadee Phenjati '94 at the Southern California ACS
Undergraduate Research Conference, LMU, April 30, 1993.
"Melting Studies of of Thrombin-Binding DNA," presented
by Fredesminda Dait '94 at the Southern California ACS
Undergraduate Research Conference, LMU, April 30, 1993.
"Calorimetric Studies of Metal-Substituted Recombinant
Myohemerythrins," Presented by Fredesminda Dait '94 and
Krisadee Phenjati '94 to The Pacific Conference, Pasadena Hilton
Hotel, October 20, 1993.
WEB JOURNALISM
"Leg One of the Labor Day Electric Vehicle Rally: From Port
Hueneme to the Pier in Santa Monica" published in September
1999 by EV1-Club.Power.Net.
Visit my personal home page at http://home.earthlink.net/~roejames/